杜洋

助理教授

教育背景

博士 (中国科学技术大学)

学士 (中南大学湘雅医学院)

研究领域
G蛋白偶联受体、受体生物学、信号转导、结构药理学、基于结构的药物设计
电子邮件
yangdu@cuhk.edu.cn
个人简介

杜洋教授2011年于中国科学技术大学取得博士学位,之后赴美国斯坦福大学医学院分子与细胞生理学系,师从2012年诺贝尔化学奖得主布莱恩·科比尔卡(Brian Kobilka)教授从事G蛋白偶联受体(GPCR)相关的博士后研究工作,并于2016年底转为斯坦福医学中心固定职位的研究科学家(Research Scientist)。期间获授美国心脏协会(American Heart Association)全额博士后奖学金和斯坦福心血管研究所资助等,并获得美国密歇根大学安娜堡分校医学院药学系助理教授职位(已婉拒)。主要研究方向是以β2肾上腺素受体(beta-2 adrenergic receptor)等重要药物靶点的G蛋白偶联受体(GPCR)为对象,研究其与下游信号分子复合物的结构、功能和分子药理特性。杜教授迄今已发表42篇高质量SCI论文(11篇第一或通讯作者论文),包括在Cell、Nature、Nature Comm.、JACS、Chem. Sci.等国际顶级期刊等报道的一系列突出的科研成果。现为香港中文大学(深圳)生命与健康科学学院助理教授、博士生导师,科比尔卡创新药物开发研究院责任研究员,同时入选广东省和深圳市高层次人才项目。

学术著作

1. He YX, Gui L, Liu YZ, Du Y, Zhou Y, Li P, Zhou CZ. Crystal structure of Saccharomyces cerevisiae glutamine synthetase Gln1 suggests a nanotube-like supramolecular assembly. Proteins. 2009, 76(1): 249-54.

2. Du Y, He YX, Gaowa S, Zhang X, Chen Y, Zhang SC, Zhou CZ. Crystal structures of the apo and GDP-bound forms of a cupin-like protein BbDUF985 from Branchiostoma belcheri tsingtauense. Proteins. 2010, 78(12): 2714-9.

3. Du Y*, He YX*, Zhang ZY, Yang YH, Shi WW, Frolet C, Di Guilmi AM, Vernet T, Zhou CZ, and Chen Y. Crystal structure of the mucin-binding protein of Spr1345 from Streptococcus pneumoniae. J. of Struct. Biol. 2011, 174: 252-257.

4. Du Y*, Shi WW*, He YX, Yang YH, Zhou CZ, Chen Y. Structures of the substrate-binding protein provide insights into the multiple compatible solutes binding specificities of Bacillus subtilis ABC transporter OpuC. Biochemical J. (2011) 436: 283-289.

5. Du Y, Cheng W, Li WF. Expression profiling reveals an unexpected growth-stimulating effect of surplus iron on the yeast Saccharomyces cerevisiae. Mol. and Cells. (2012) 34: 127-132.

6. Carr R 3rd, Du Y, Quoyer J, Panettieri RA Jr, Janz JM, Bouvier M, Kobilka BK, Benovic JL. Development and characterization of pepducins as Gs-biased allosteric agonists. J. Biol. Chem. (2014) 289: 35668-84.

7. Cho KH, Du Y, Scull NJ, Hariharan P, Gotfryd K, Loland CJ, Guan L, Byrne B, Kobilka BK, Chae PS. Novel Xylene-Linked Maltoside Amphiphiles (XMAs) for Membrane Protein Stabilisation. Chemistry. (2015) 21: 10008-13.

8. Hussain H, Du Y, Scull NJ, Mortensen JS, Tarrasch J, Loland CJ, Bernadette Byrne B, Kobilka BK, and Chae PS. Accessible mannitol-based amphiphiles (MNAs) for membrane protein solubilisation and stabilization. Chemistry. (2016) 22: 7068-73.

9. Tian X, Irannejad R, Bowman SL, Du Y, Puthenveedu MA, Zastrow M, and Benovic JL. The α-Arrestin ARRDC3 Regulates the Endosomal Residence Time and Intracellular Signaling of the β2-Adrenergic Receptor. J. Biol. Chem. (2016) 291: 14510-25.

10. DeVree B, Mahoney J, Velez-Ruiz G, Rasmussen SGF, Kuszak A, Edwald E, Fung JJ, Manglik A, Masureel M, Du Y, Matt R, Pardon E, Steyaert J, Kobilka BK, Sunahara RK. Allosteric coupling from G protein to the agonist binding pocket in GPCRs. Nature. (2016) 535 (7610):182-6.

11. Carr R 3rd, Schilling J, Song J, Carter RL, Du Y, Yoo SM, Cheung JY, Tilley DG, Benovic JL. β-arrestin-biased signaling through the β2-adrenergic receptor promotes cardiomyocyte contraction. Proc. Natl. Acad. Sci. (2016) 113: 4107-16.

12. Cho KH, Hariharan P, Mortensen JS, Du Y, Nielsen AK, Byrne B, Kobilka BK, Loland CJ, Guan L, Chae PS. Isomeric detergent comparison for membrane proteins stability: importance of inter alkyl chain distance and alkyl chain length. ChemBioChem (2016) 17:2334-2339.

13. Bae HE, Mortensen JS, Ribeiro O, Du Y, Ehsan M, Kobilka BK, Loland CJ, Byrne B, Chae PS. Tandem neopentyl glycol maltosides (TNMs) for membrane protein stabilization. Chemical Communications (2016) 52: 12104-12107.

14. Cho KH, Scull NJ, Du Y, Hariharan P, Mortensen JS, Loland CJ, Guan L, Kobilka BK, Byrne B, Chae PS. Mesitylene-cored glucoside amphiphiles (MGAs) for membrane protein study: importance of alkyl chain density in detergent efficacy. Chemistry (2016) 10.1002/chem.201603338.

15. Das M, Du Y, Mortensen JS, Ribeiro O, Loland CJ, Kobilka BK, Byrne B, Chae PS. Butane-1,2,3,4-tetraol-based Amphiphilic Stereoisomers for Membrane Protein Study: Importance of Chirality in the Hydrophobic Region. Chemical Science (2017) 8: 1169-1177.

16. Woldring DR, Holec PV, Stern LA, Du Y, Hackel, BJ. A gradient of sitewise diversity promotes evolutionary fitness for binder discovery in a three-helix bundle protein scaffold. Biochemistry (2017) 56: 1656-1671.

17. Hussain H, Mortensen JS, Du Y, Santillan C, Ribeiro O, Go J, Loland CJ, Guan L, Kobilka BK, Byrne B, Chae PS. Tandem malonate-based glucosides (TMGs) for membrane protein structural study. Scientific Reports (2017) doi: 10.1039/c6cc06147h.

18. Hussain H, Du Y, Tikhonova E, Mortensen JS, Ribeiro O, Santillan C, Das M, Loland CJ, Guan L, Kobilka BK, Byrne B, Chae PS. Resorcinarene-based facial glycosides: implication of detergent flexibility on membrane protein stability. Chemistry (2017) doi: 10.1002/chem.201605016.

19. Ehsan M, Ghani L, Du Y, Mortensen JS, Ribeiro O, Hu H, Skiniotis G, Loland CJ, Kobilka BK, Byrne B, Chae PS. New penta-saccharide-bearing tripod amphiphiles for membrane protein structure studies. Analyst (2017) 142: 3889-3898.

20. Ehsan M, Du Y, Molist I, Seven AB, Hariharan P, Mortensen JS, Ghani L, Loland CJ, Skiniotis G, Guan L, Byrne B, Kobilka BK, Chae PS. Vitamin E-based glycoside amphiphiles for membrane protein structural studies. Organic and Biomolecular Chemistry (2018) DOI: 10.1039/c8ob00270c.

21. Ehsan M, Das M, Stern V, Du Y, Mortensen JS, Hariharan P, Byrne B, Loland CJ, Kobilka BK, Guan L, Chae PS. Steroid-based amphiphiles for membrane protein study: Importance of alkyl spacer for protein stability. ChemBioChem (2018) doi: 10.1002/cbic.201800106.

22. Das M, Du Y, Mortensen JS, Bae HE, Byrne B, Loland CJ, Kobilka BK, Chae PS. An engineered lithocholate-based facial amphiphile stabilizes membrane proteins: assessing the impact of detergent modularity on protein stability. Chemistry (2018), accepted.

23. Das M, Du Y, Mortensen JS, Hariharan P, Lee HS, Byrne B, Loland CJ, Guan L, Kobilka BK, Chae PS. Rationally engineered tandem facial amphiphiles for improved membrane protein stabilization efficacy. ChemBioChem (2018) Accepted.

24. Duc NM*, Du Y* (* co-first author), Thorsen TS, Lee SY, Zhang C, Kato H, Kobilka BK, Chung KY. Effective Application of Bicelles for Conformational Analysis of G Protein-Coupled Receptors by Hydrogen/Deuterium Exchange Mass Spectrometry. J. Am. Soc. Mass. Spectrom. (2015) 26: 808-817.

25. Sadaf A*, Du Y* (* co-first author), Hariharan P, Mortensen JS, Perez IM, Seven AB, Santillan C, Skiniotis G, Loland CJ, Kobilka BK, Guan L, Byrne B, Chae PS. Dendronic trimaltoside amphiphiles (DTMs) for membrane protein structure study. Chemical Science (2017) 8: 1169-1177.

26. Ehsan M*, Du Y* (* co-first author), Scull NJ, Tikhonova E, Tarrasch J, Mortensen JS, Loland CJ, Skiniotis G, Guan L, Byrne B, Kobilka BK, Chae PS. Highly Branched Penta-Saccharide-Bearing Amphiphiles for Membrane Protein Studies. J. Am. Chem. Soc. (2016) 138: 3789-96.

27. Ehsan M, Kumar A, Mortensen JS, Du Y, Hariharan P, Byrne B, Guan L, Kobilka BK, Loland CJ, Chae PS. Self-assembly behaviors of a penta-phenylene maltoside and its application for membrane protein study. Chemistry-An Asian Journal (2019) Apr 10. doi: 10.1002/asia.201900224.

28. Chae PS, Ehsan M, Du Y, Mortensen JS, Hariharan P, Qu Q, Ghani L, Das M, Grethen A, Byrne B, Skiniotis G, Keller S, Loland CJ, Guan L, Kobilka BK. Self-assembly behaviors and application of terphenyl-cored trimaltosides for membrane protein study: Impact of detergent hydrophobic group geometry on protein stability. Chemistry (2019) Jun 26. doi: 10.1002/chem.201902468.

29. Sadaf A, Ramos M, Mortensen JS, Du Y, Bae HE, Munk CF, Hariharan P, Byrne B, Kobilka BK, Loland CJ, Guan L, Chae PS. Conformationally restricted monosaccharide-cored glycoside amphiphiles: the effect of detergent head group variation on membrane protein stability. ACS Chem Biol (2019) Jul 15. doi: 10.1021/acschembio.9b00166.

30. Strohman MJ, Maeda S, Hilger D, Masureel M, Du Y, Kobilka BK. Local membrane charge regulates β2 adrenergic receptor-Gi interaction. Nature Communications (2019) May 20;10(1):2234.

31. Ghani L, Munk C, Zhang X, Katsube S, Du Y, Cecchetti C, Huang WJ, Hyoung B, Saouros S, Ehsan M, Guan L, Liu XY, Loland C, Kobilka BK, Byrne B, Chae PS. 1,3,5-Triazine-cored maltoside amphiphiles for membrane protein extraction and stabilization. Journal of the American Chemical Society (2019) Dec 18; 141(50):19677-87.

32. Duc NM*, Du Y* (* co-first author), Thorsen TS, Lee SY, Zhang C, Kato H, Kobilka BK, Chung KY. Effective Application of Bicelles for Conformational Analysis of G Protein-Coupled Receptors by Hydrogen/Deuterium Exchange Mass Spectrometry. J. Am. Soc. Mass. Spectrom. (2015) 26: 808-817.

33. Sadaf A*, Du Y* (* co-first author), Hariharan P, Mortensen JS, Perez IM, Seven AB, Santillan C, Skiniotis G, Loland CJ, Kobilka BK, Guan L, Byrne B, Chae PS. Dendronic trimaltoside amphiphiles (DTMs) for membrane protein structure study. Chemical Science (2017) 8: 1169-1177.

34. Ehsan M*, Du Y* (* co-first author), Scull NJ, Tikhonova E, Tarrasch J, Mortensen JS, Loland CJ, Skiniotis G, Guan L, Byrne B, Kobilka BK, Chae PS. Highly Branched Penta-Saccharide-Bearing Amphiphiles for Membrane Protein Studies. J. Am. Chem. Soc. (2016) 138: 3789-96.

35. Das M*, Du Y* (* co-first author), Ribeiro O, Hariharan P, Mortensen JS, Patra D, Skiniotis G, Loland CJ, Guan L, Kobilka BK, Byrne B, Chae, PS. Conformationally preorganized diastereomeric norbornane-based maltosides (NBMs) for membrane protein study: Implications of detergent kink for micellar properties. J. Am. Chem. Soc. (2017) 139: 3072-81.

36 Komolov KE*, Du Y* (* co-first author), Duc NM, Betz R, Rodrigues J, Leib RD, Patra D, Skiniotis G, Adams CM, Dror R, Chung KY, Kobilka BK, Benovic JL. Structural and functional analysis of a β2-Adrenergic Receptor Complex with GRK5. Cell (2017) 169: 407-421 –Selected as the Featured Article of the Issue Recommended by F1000Prime: f1000.com/prime/727537208.

37. Liu X, Xu X, Hilger D, Aschauer P, Tiemann JKS, Du Y, Liu H, Hirata K, Sun X, Guixa-Gonzalez R, Mathiesen JM, Hidebrand PW, Kobilka BK. Structural insights into the process of GPCR-G protein complex formation. Cell (2019) 177(5):1243-1251.

38. Du Y*, Duc NM*, Rasmussen SGF*, Hilger D, Kubiak X, Wang L, Kim HR, Wegrecki M, Asuru A, Jeong KM, Lee J, Chance M, Lodowski DT, Kobilka BK, Chung KY. Assembly of a GPCR-G protein complex. Cell (2019) 177(5):1232-1242.